オリジナルのBL21-CodonPlus ‐RIL and RP 株はそれぞれAT-および GC-リッチのゲノム用に最適化されました。ご自身の配列のコドン使用量がお分かりの場合は、これらの菌株をご利用下さい。
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Codon Usage in Various Organisms Codon frequencies are expressed as codons used per 1000 codons encountered. The arginine codons AGG and AGA are recognized by the same tRNA and should therefore be combined. Codon frequencies of more than 15 codons/1000 codons are shown in bold to help identify a codon bias that may cause problems for high-level expression in E. coli. These frequencies are updated regularly. A complete compilation of codon usage of the sequences in the GenBank database can be found at www.kazusa.or.jp/codon/.
Superior Expression of Sequences with Codon Bias The human cardiac troponin-T gene (hcTnT) (argU dependent) and two test genes, CBP-3xI-Cre (ileW dependent) and CBP-3xP-Cre (proL dependent) were cloned into T7 expression vectors and expressed in either parental BL21(DE3) or BL21-CodonPlus(DE3)-RIPL competent cells. As shown, BL21-CodonPlus(DE3)-RIPL cells dramatically improve expression of proteins by overcoming codon bias compared to traditional BL21(DE3) cells and rescue expression of both AT- and GC-rich genomes.
Codon usage: Purchase of this product is accompanied by a limited use license to use the product as a reagent for research purposes. If Purchaser intends to use the product outside the scope of the license, he needs to enter into a License Agreement with F. Hoffmann-La Roche Ltd, Grenzacherstrasse 124, 4070 Basel, Switzerland or Hoffmann-La Roche Inc.
These cells contain extra copies of rare E. coli argU, ileY, leuW, proL tRNA genes which corrects for codon bias and dramatically improves expression of sequences from other organisms.
BL21-CodonPlus(DE3)-RIPL competent cells, pUC18 control DNA, XL10-GoldÒ BME